Count 96-spot plates or traditional round plates
CIRCUS
The Circus feature in Count-On-It is a convenient tool for counting standard agar plates. Colonies are detected by brightness, which is enhanced when using fluorescent filter sets, and can be further distinguished by selecting from a single channel. Circus is well suited to batch processing folders of plate images, it produces a .csv file with the colony count and associate notes, as well a a graphic 'receipt' depicting the counting method. Circus is capable of segmenting tightly packed colonies, and is therefore capable of accurately counting thousands of colonies on a single plate, depending on colony size.
Gridiron
The Gridiron feature of Count-On-It is custom built for counting 96-spot plates. This approach measures CFUs by placing droplets of inoculum in a grid using a 96-channel pipette, enabling up to 96 samples to be grown on a single rectangular plate. Gridiron generates a results table the the colony count for each cell on the plate, and also produces a 'receipt' which is used to visualize, verify and record the counting results. Serial dilutions can be across multiple plates or within a single plate. The software is well suited to batch processing images.